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Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development

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After centrifugation (1200 g, 10 min), the supernatant was discarded, and the resulting cell pellet was resuspended in culture medium supplemented with 5% of (FBS) (F4135, Sigma- Aldrich, Pozna, Poland) and seeded into cell culture flasks

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At the starting dose of 2.5mg per week, a 10mg vial lasts four weeks